Facility User Agreement Titan Krios Electron Microscope

This is a 300keV instrument with an X-FEG electron source, equipped with a Gatan Quantum energy filter, a K2 Summit direct electron detector and Volta Phase Plates. The instrument also has STEM capabilities. Capable of performing single-particle or tomography data collections utilizing Leginon, EPU, and SerialEM (tomography) automation packages.

Prerequisites for independent use of the Krios requires completion of the following training path or
demonstrated proficiency:

• New User Orientation
• Negative Stain and Wet Lab
• Sphera Training
• Autoloader Clipping and Loading
• Titan Krios Training
  • 2 – 4 hour one on one sessions with facility staff
  • 5 – 2 hour observations sessions with an independent user
  • 100 hours of supervised instrument time logged
  • Final approval by facility staff by demonstration of proficiency

Rules

• Reservations can be made 30 days in advance
• Cancellations allowed up to 48 hours before the reservation time (Cancellation charges waived if time can be rebooked)
• Reservations limited to 48 hours maximum
• Users must log in to FOM upon arrival and start of work
• Users must log off in FOM upon completion of work
• Independent use charged to the nearest hour
• Charges will be based on earlier of reserved or actual start time – later of reserved or actual end
• No shows will be charged for reserved time
• Damage to equipment due to negligence or improper use will be charged to the originating lab
• All data is offloaded to an external server. Users must coordinate with SDSC or PCF for retrieval of data.
• Sample exchanges are performed at 10AM sharp. If previous user of the scope is not present and no arrangement has been made prior, the incoming user may discard the previous user’s grids
• Facility staff is not responsible for any storage of samples.

Recharge Rates

• Internal Collaborative Use:   $186.00/hr
• Internal Supervised Use:   $136.00/hr
• Internal Independent Use:   $86.00/hr