Training Modules Offered
Training costs are calculated based on staff time plus time spend on the instrument of choice. Staff time is charged at $100/hour, instrument rates vary.
Negative Staining and Wet lab
Description: Offered for specific cases only, since it is often unnecessary for structural cryo-EM work.
Duration: 2 hours session with EM facility staff.
Occupancy: Maximum 2 person.
Supplies needed (available for purchase from the EM facility store): Grid box, continuous carbon 300 mesh grids, tweezers.
Safematic Carbon Coater
Description: Training on using the Safematic carbon coater, practical application, recipe creation.
Duration: 1 hour session from EM facility staff.
Occupancy: Maximum 2 person.
Supplies needed: Grids for coating, mica (provided by facility).
Vitrobot
Description: Training on using the Vitrobot, cryo sample preparation theory, setup, practical application, use of Solarus plasma cleaner/glow discharge.
Duration: 2 separate 2-hour sessions with EM facility staff member.
Occupancy: Maximum 2 person.
Supplies needed (available for purchase from the EM facility store): Autogrid box with lid, quantifoil grids, storage for cryo grids. Users must supply their own tweezers (tweezers can be provided during training). Recommended tweezers options: Ted Pella vitrobot tweezers or NanoSoft cryo tweezers.
Manual Plunge freezer
Description: Training on using the manual plunge freezer.
Duration: 2 hour session with EM facility staff.
Occupancy: Maximum 2 person.
Supplies needed (available for purchase from the EM facility store): Autogrid box with lid, filter paper (whatman #1), quantifoil grids, plunge freezer tweezers, storage for cryo grids.
Autogrid Clipping
Description: Training on clipping cryo-EM grids for Krios or Tundra samples.
Duration: 2 hour session, 1 hour demonstration from EM facility staff and 1 hour practice
Occupancy: Maximum 1 person.
Supplies needed (available for purchase from the EM facility store): Autogrid box with lid, cryo-EM grids, autogrid rings and c-clips, storage for cryo grids.
Krios Electron Microscope
Description: Training and hands-on practical safe use of Krios for screening cryo grids and setting up data collection.
Duration: 2 separate training sessions with EM facility staff.
Occupancy: Maximum 1-2 persons.
Supplies needed: Vitrified autogrids
Tundra Electron Microscope
Description: Training and hands-on practical safe use of Tundra for screening negative staining grids and cryo grids and setting up data collection.
Duration: 2 separate training sessions with EM facility staff.
Occupancy: Maximum 1-2 persons.
Supplies needed: Negative stained or vitrified autogrids
